Unlock the Secrets of Cellular Biology

Exploring the Complexities of Centrosome and Cilia Biology

About the CCB lab
Welcome to the Laboratory of Centrosome and Cilia Biology! Our state-of-the-art laboratory is dedicated to the study of centrosomes and cilia, crucial organelles involved in various cellular processes. Led by a team of passionate researchers, we are committed to unraveling the mysteries of centrosome and cilia biology, paving the way for groundbreaking discoveries and potential therapeutic applications. Explore our website to learn about our ongoing projects, recent publications, and opportunities for collaboration.
About The PI
Dr. Karthigeyan Dhanasekaran
Assistant Professor
Centrosome and Cilia biology lab

  • BVSc & AH, Madras Veterinary College, Chennai, 1999-2005
  • MVSc (Molecular Biology and Biotechnology), GBPUAT, Pantnagar, 2005-2007.
  • PhD (Molecular Biology and Genetics), JNCASR, Bangalore,2007-2013.
  • Post-doctoral Research at the Yale School of Medicine, USA, 2015-2020.
  • Assistant Professor at the RCB, Faridabad since 2020.
  • Ramalingaswami Fellow since 2021.
Cutting-Edge Research
Our research interests lie in understanding the perturbation of centriole based organelles across varying disease states that contribute to the pathobiological manifestations and how best to intervene them to restore the physiology. We are trying to understand the centrosome and cilia using high resolution imaging based approaches and focusing on their signalling roles by means of biochemical and molecular cell biology approaches combined with modern proteomics, electron microscopy and super-resolution microscopy based tools.

Area of interest

Centrosome and Ciliary defects

Structural and functional perturbations of Centriolar and Cytoskeletal elements contributing to disease pathology.

Centriolar Signalosomes

Signalosomes operating across centrosome and ciliary basal bodies in infection and ageing.

Centriolar DNA damage response.

Centrosomal response in sensing and responding to DNA damage.

Few more interests

Cytoskeletal Rearrangement

Mechanisms leading to cytoskeletal rearrangements during viral infection.

Centrosome Amplification

Cause and effects of supernumerary centrosomes.

Non canonical role of Aurora A Kinase

Extra mitotic role over the centrosome.

Team

Himanshi Amita

PhD Scholar SRF

Exploring centrosome targeting mechanism of RNA viral proteins and studying the centriolar perturbations in the infected host cell using molecular and cellular approaches. Apart from research I like to draw mandala art in my free time.

Manish Yadav

PhD Scholar JRF

Investigating the mechanism underlying centriolar and cytoskeletal dynamics during RNA viral infection. In my Free time I enjoy playing cricket and the Joy of the Open Road! A New Car Experience.

Sanyami Jain

PhD Scholar JRF

Working to understand DNA damage response in the context of centrosome, with a focus on the role of Aurora kinase A in this process, using molecular and cellular approaches. When I'm not in lab, I enjoy baking, reading and watching movies.

Heshica Bhattina

MSc Scholar

Investigating the role of centrosome dysregulation in Type 2 diabetes and its impact on other ageing disorders. Beyond the lab, I'm a nature enthusiast who finds serenity in the great outdoors. I'm also passionate about expressing myself through art, finding solace in the strokes of painting and drawing.

Team

Pranay Dudhe

Technical Support for Bio-Imaging

Contributing to the cutting-edge field of Super-resolution imaging, facilitating breakthroughs in understanding cellular structures and dynamics, by employing innovative methodologies and image processing. I have a deep passion for photography, cooking, and swimming, and indulging in the world of movies is another source of joy for me.

Kanak

Project Assistant

"Revealing centrosome biology in Parkinson's disease" . Wordsmith by hobby, weaving science and philosophy into anthologies.

Vrushalee Mhaske

Project Assistant

I am interested in understanding the CHIKV’s impact on host cytoskeletal reorganization during viral lifecycle through molecular virology, imaging and biochemical approaches. Apart from research, I enjoy performing Lavani and playing Lawn Tennis.

Sanjay Kumar

Lab Assistant

I enjoy playing Volley ball and Cricket after work.

Timeline

 

First student joining

Himanshi joins the team initiating our Flaviviral study

5/4/2021
 

Zidhan joined our team

Kick starts the viral cell biology in our team

1/2/2022
 

Time 3

Tulasiram Joined our team for an exciting gene editing role

28/11/2022
 

Manish Joins CHIKV project to pursue his PhD & Pranay joins our team with great passion

3/10/2023

Farewell to Zidhan

Ends on a high note with some novel observations documented beyond mere discovery.

30/4/2024

Farewell to Tulasiram

One more long-term association that will be remembered by the team in future.

31/1/2025

Alumni

Zidhan Subair

Project Assistant

Understanding the CHIKV host cell biology by means of molecular virology, cellular imaging and subcellular visualisation. Outside the lab I enjoy a good book or movie and relish tasting new food.

Tulasiram Mora

Project Assistant

Designing tools to explore centrosomes and beyond using gene editing and stable cell line generation. Apart from science, I like to watch movies.

Aparna Upadrasta

Project JRF

Molecular signatures defining centrosome localization and retention using molecular and proteomic approaches. I like to spend my free time solving Sudoku, keeping up with world geopolitics and learning Carnatic music if I'm not in the lab.

Neha Sharma

Project Assistant

Understanding the contributions of liquid-liquid phase separation (LLPS) in centrosome targeted proteins during ageing and infection. I like expressing myself through writing.

Publications

 Betrayal From the Core: Centriolar and Cytoskeletal Subversion by Infectious Pathogens. 

Himanshi Amita, Zidhan Subair, Tulasiram Mora, Pranay Eknath Dudhe, Karthigeyan Dhanasekaran: First published: 04 February 2025 https://doi.org/10.1002/cm.22004
     

 Imaging of intracellular protein aggregates through plasmon-assisted clusteroluminescence. Nanoscale:  2024, 16, 11749-11761. 

Ashish Kumar Kumar Dhillon,   Pranay Eknath Dudhe,   Shubhangi Majumdar,   Sanmitra Barman,   Dibyajyoti Ghosh*,   Karthigeyan Dhanasekaran* and  Soumik Siddhanta*: Authors
     

 The LEM-ESCRT toolkit: Repair and maintenance of the nucleus. (2022) Frontiers Cell & Developmental Biology (10), 989217. (Review Article) 

*Borah S, *Dhanasekaran K, *Balot S: Authors

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Testimonials

     

 The team's expertise and dedication have exceeded my expectations. Their detailed reports, scientifically engaging discussions and speedy turnaround have been invaluable to our scientific career. 

Bharat Gurnani: MSc Dissertation Trainee
     

 During my stay at CCB, I got valuable exposure and expertise in the subject of genetic engineering, as well as inspiration to continue forward and further improve my talents. Every day questions kept me interested and helped me better comprehend the strategies. Dr. Karthik was constantly available to students, leading, challenging, and clarifying doubts while creating goals for daily self-learning. Apart from the academic presentations, the lab environment was pleasant and welcoming, which made it simpler for me to ask questions, clarify, and engage in constructive discussions. I am prepared to take my laboratory expertise with me wherever I go on my academic research path in biotechnology. 

Bani Preet: Summer Trainee
     

 I had all the opportunity to expand my knowledge and skills during my training program. I truly appreciate your support and guidance throughout my academic journey, and I look forward to applying what I have learned under your mentorship. 

Shankar S: Dissertation Trainee
  

Gallery

Contact


 karthigeyan@rcb.res.in

  • REGIONAL CENTRE FOR BIOTECHNOLOGY, 3rd Milestone, Faridabad - Gurgaon Road, Faridabad, Haryana, India
  • CCB LAB, Dept of Cancer & Cell Biology
  •  Mon-Fri - 08:00-19:00